研究进展-唐宏武课题组

Optical trapping of a single virus in a host cell based on active-passive calibration for trap stiffness

Abstract: Optical tweezers are considered a tool for trapping and measuring forces on single molecules and small particles. Current research mainly focuses on measuring force spectral information of particles suspended in solution. However, the viscoelastic environment within the cell presents additional challenges for the in situ calibration of force spectroscopy data. To address this issue, we used ...

2025-05-16

Optical Tweezer-Driven Mechanotransduction: Probing pN-Scale Forces and Calcium-Mediated Redox Signaling in Single Endothelial Cells

Abstract: Endothelial cells (ECs) regulate vascular function by converting mechanical forces into biochemical signals; however, the molecular mechanisms of pN-scale mechanotransduction remain elusive. Here, we develop an optical tweezer-integrated confocal microscopy system that allows precise, noninvasive manipulation of the cell membrane localization with mechanical stimuli within the 0–100 pN range ...

2025-04-08

Tunneling nanotubes mediate KRas transport: Inducing tumor heterogeneity and altering cellular membrane mechanical properties

Abstract: Mutation in oncogene KRas plays a crucial role in the occurrence and progression of numerous malig- nant tumors. Malignancy involves changes in cell mechanics for extensive cellular deformation during metastatic dissemination. We hypothesize that oncogene KRas mutations are intrinsic to alterations in cellular mechanics that promote malignant tumor generation and progression. Here, we demonstrate ...

2024-06-27

Aggregation-Induced Emission (AIE) of Au(I)-GSH Complexes is Activated by Optical Manipulation and Exhibits Surprising 780 nm Emission

Abstract: An optical manipulation method to activate the aggregation-induced emission (AIE) of Au(I)-glutathione (Au(I)-GSH) complexes is presented. First, the well-known AIE-type Au(I)-GSH complexes with a solid emission wavelength of 570 nm are synthesized. At the beginning of the focusing a 1064 nm continuous-wave (CW) laser at the interface of Au(I)-GSH complexes solution, its luminescence is hardly ...

2024-01-15

Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palindromic Repeats-Cas12a Based on Au Nanoparticle-DNAzyme Probe and On-Site Detection of Pb2+ Via the Photonic Crystal Chip

Abstract: Although great headway has been made in DNAzyme-based detection of Pb2+, its adaptability, sensitivity, and accessibility in complex media still need to be improved. For this, we introduce new ways to surmount these hurdles. First, a spherical nucleic acid (SNA) fluorescence probe (Au nanoparticles-DNAzyme probe) is utilized to specifically identify Pb2+ and its suitability for precise detection ...

2024-01-01

Boosting optical trapping a single virus by quantum dots tagging increases virus polarizability and trap stiffness

Abstract: Abstract：Optical tweezers use the momentum of photons to capture and manipulate particles in a noncontact way. Although related techniques have been widely used in biology and materials, the research on viruses is still relatively limited. It is hard to optically trap viruses because trap stiffness is rather low and the size of viruses are too small. Here, we used an optical tweezers system ...

2023-11-15